human dlbcl cell lines u-2932 Search Results


abc dlbcl u 2932 dsmz  (DSMZ)
95
DSMZ abc dlbcl u 2932 dsmz
Abc Dlbcl U 2932 Dsmz, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dlbcl cells  (ATCC)
95
ATCC human dlbcl cells
The reciprocal repression effect of LINC00908 and miR-671-5p. ( A ) Subcellular fractionation assay was used to determine the subcellular localization of LINC00908. ( B ) Expression levels of miR-671-5p <t>in</t> <t>U2932</t> and FARAGE cells after the knockdown of LINC00908. ( C ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p mimic. ( D ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p inhibitor. ( E ) Expression of miR-671-5p in 28 <t>DLBCL</t> tissues and paired normal tissues based on qRT-PCR. ( F ) The association between LINC00908 and miR-671-5p was examined. ( G ) Expression of miR-671-5p in the GB12878 cells and DLBCL cell lines based on qRT-PCR. **p < 0.01.
Human Dlbcl Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dlbcl cell lines  (ATCC)
95
ATCC human dlbcl cell lines
The reciprocal repression effect of LINC00908 and miR-671-5p. ( A ) Subcellular fractionation assay was used to determine the subcellular localization of LINC00908. ( B ) Expression levels of miR-671-5p <t>in</t> <t>U2932</t> and FARAGE cells after the knockdown of LINC00908. ( C ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p mimic. ( D ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p inhibitor. ( E ) Expression of miR-671-5p in 28 <t>DLBCL</t> tissues and paired normal tissues based on qRT-PCR. ( F ) The association between LINC00908 and miR-671-5p was examined. ( G ) Expression of miR-671-5p in the GB12878 cells and DLBCL cell lines based on qRT-PCR. **p < 0.01.
Human Dlbcl Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diffuse large b cell lymphoma dlbcl derived cell lines  (DSMZ)
94
DSMZ diffuse large b cell lymphoma dlbcl derived cell lines
The reciprocal repression effect of LINC00908 and miR-671-5p. ( A ) Subcellular fractionation assay was used to determine the subcellular localization of LINC00908. ( B ) Expression levels of miR-671-5p <t>in</t> <t>U2932</t> and FARAGE cells after the knockdown of LINC00908. ( C ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p mimic. ( D ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p inhibitor. ( E ) Expression of miR-671-5p in 28 <t>DLBCL</t> tissues and paired normal tissues based on qRT-PCR. ( F ) The association between LINC00908 and miR-671-5p was examined. ( G ) Expression of miR-671-5p in the GB12878 cells and DLBCL cell lines based on qRT-PCR. **p < 0.01.
Diffuse Large B Cell Lymphoma Dlbcl Derived Cell Lines, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dlbcl cell lines  (DSMZ)
95
DSMZ dlbcl cell lines
Figure 2. Iron chelators and ironomycin inhibit <t>DLBCL</t> <t>cell</t> growth and decrease the LIP. A–C, DLBCL cells were incubated with different concentrations of iron chelators or vehicle for 96 hours. IC50 was calculated with concentration–response curve after treatment with deferoxamine (A), deferasirox (B), and ironomycin (C). D, Cell viability was examined using quantification of ATP assay. Data are expressed as mean percentage SEM of at least three independent experiments performed in six times. Balb/c mice were inoculated with murine A20 lymphoma cells and when tumor was palpable, the mice were treated with ironomycin (3 mg/kg i.p.). Mice were sacrificed when tumor volume reached 1,500 mm3. Evaluation of the tumor volume of vehicle (n ¼ 10) and ironomycin-treated mice (n ¼ 10). , P < 0.05 using Mann– Whitney test. E, Effect of Ironomycin on LIP was measured by dequenching of Calcein. Cells were loaded with 0.25 mmol/L Calcein-AM for 15 min, washed, and incubated for 1 hour with or without 100 mmol/L deferasirox or 10 mmol/L ironomycin. In this experiment, following treatment, cells were washed and their fluorescence was measured by flow cytometry. The difference in the mean fluorescence index between chelator-treated and untreated cells (DF) is presented. The results represent the mean and SD of at least four independent experiments.
Dlbcl Cell Lines, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human abc dlbcl cell lines  (ATCC)
95
ATCC human abc dlbcl cell lines
Figure 2. Iron chelators and ironomycin inhibit <t>DLBCL</t> <t>cell</t> growth and decrease the LIP. A–C, DLBCL cells were incubated with different concentrations of iron chelators or vehicle for 96 hours. IC50 was calculated with concentration–response curve after treatment with deferoxamine (A), deferasirox (B), and ironomycin (C). D, Cell viability was examined using quantification of ATP assay. Data are expressed as mean percentage SEM of at least three independent experiments performed in six times. Balb/c mice were inoculated with murine A20 lymphoma cells and when tumor was palpable, the mice were treated with ironomycin (3 mg/kg i.p.). Mice were sacrificed when tumor volume reached 1,500 mm3. Evaluation of the tumor volume of vehicle (n ¼ 10) and ironomycin-treated mice (n ¼ 10). , P < 0.05 using Mann– Whitney test. E, Effect of Ironomycin on LIP was measured by dequenching of Calcein. Cells were loaded with 0.25 mmol/L Calcein-AM for 15 min, washed, and incubated for 1 hour with or without 100 mmol/L deferasirox or 10 mmol/L ironomycin. In this experiment, following treatment, cells were washed and their fluorescence was measured by flow cytometry. The difference in the mean fluorescence index between chelator-treated and untreated cells (DF) is presented. The results represent the mean and SD of at least four independent experiments.
Human Abc Dlbcl Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oci ly 19  (DSMZ)
95
DSMZ oci ly 19
Figure 2. Iron chelators and ironomycin inhibit <t>DLBCL</t> <t>cell</t> growth and decrease the LIP. A–C, DLBCL cells were incubated with different concentrations of iron chelators or vehicle for 96 hours. IC50 was calculated with concentration–response curve after treatment with deferoxamine (A), deferasirox (B), and ironomycin (C). D, Cell viability was examined using quantification of ATP assay. Data are expressed as mean percentage SEM of at least three independent experiments performed in six times. Balb/c mice were inoculated with murine A20 lymphoma cells and when tumor was palpable, the mice were treated with ironomycin (3 mg/kg i.p.). Mice were sacrificed when tumor volume reached 1,500 mm3. Evaluation of the tumor volume of vehicle (n ¼ 10) and ironomycin-treated mice (n ¼ 10). , P < 0.05 using Mann– Whitney test. E, Effect of Ironomycin on LIP was measured by dequenching of Calcein. Cells were loaded with 0.25 mmol/L Calcein-AM for 15 min, washed, and incubated for 1 hour with or without 100 mmol/L deferasirox or 10 mmol/L ironomycin. In this experiment, following treatment, cells were washed and their fluorescence was measured by flow cytometry. The difference in the mean fluorescence index between chelator-treated and untreated cells (DF) is presented. The results represent the mean and SD of at least four independent experiments.
Oci Ly 19, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sudhl 6  (DSMZ)
94
DSMZ sudhl 6
Figure 2. Iron chelators and ironomycin inhibit <t>DLBCL</t> <t>cell</t> growth and decrease the LIP. A–C, DLBCL cells were incubated with different concentrations of iron chelators or vehicle for 96 hours. IC50 was calculated with concentration–response curve after treatment with deferoxamine (A), deferasirox (B), and ironomycin (C). D, Cell viability was examined using quantification of ATP assay. Data are expressed as mean percentage SEM of at least three independent experiments performed in six times. Balb/c mice were inoculated with murine A20 lymphoma cells and when tumor was palpable, the mice were treated with ironomycin (3 mg/kg i.p.). Mice were sacrificed when tumor volume reached 1,500 mm3. Evaluation of the tumor volume of vehicle (n ¼ 10) and ironomycin-treated mice (n ¼ 10). , P < 0.05 using Mann– Whitney test. E, Effect of Ironomycin on LIP was measured by dequenching of Calcein. Cells were loaded with 0.25 mmol/L Calcein-AM for 15 min, washed, and incubated for 1 hour with or without 100 mmol/L deferasirox or 10 mmol/L ironomycin. In this experiment, following treatment, cells were washed and their fluorescence was measured by flow cytometry. The difference in the mean fluorescence index between chelator-treated and untreated cells (DF) is presented. The results represent the mean and SD of at least four independent experiments.
Sudhl 6, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lines sudhl 4  (DSMZ)
94
DSMZ cell lines sudhl 4
Figure 2. Iron chelators and ironomycin inhibit <t>DLBCL</t> <t>cell</t> growth and decrease the LIP. A–C, DLBCL cells were incubated with different concentrations of iron chelators or vehicle for 96 hours. IC50 was calculated with concentration–response curve after treatment with deferoxamine (A), deferasirox (B), and ironomycin (C). D, Cell viability was examined using quantification of ATP assay. Data are expressed as mean percentage SEM of at least three independent experiments performed in six times. Balb/c mice were inoculated with murine A20 lymphoma cells and when tumor was palpable, the mice were treated with ironomycin (3 mg/kg i.p.). Mice were sacrificed when tumor volume reached 1,500 mm3. Evaluation of the tumor volume of vehicle (n ¼ 10) and ironomycin-treated mice (n ¼ 10). , P < 0.05 using Mann– Whitney test. E, Effect of Ironomycin on LIP was measured by dequenching of Calcein. Cells were loaded with 0.25 mmol/L Calcein-AM for 15 min, washed, and incubated for 1 hour with or without 100 mmol/L deferasirox or 10 mmol/L ironomycin. In this experiment, following treatment, cells were washed and their fluorescence was measured by flow cytometry. The difference in the mean fluorescence index between chelator-treated and untreated cells (DF) is presented. The results represent the mean and SD of at least four independent experiments.
Cell Lines Sudhl 4, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dlbcl  (DSMZ)
90
DSMZ dlbcl
Figure 2. Iron chelators and ironomycin inhibit <t>DLBCL</t> <t>cell</t> growth and decrease the LIP. A–C, DLBCL cells were incubated with different concentrations of iron chelators or vehicle for 96 hours. IC50 was calculated with concentration–response curve after treatment with deferoxamine (A), deferasirox (B), and ironomycin (C). D, Cell viability was examined using quantification of ATP assay. Data are expressed as mean percentage SEM of at least three independent experiments performed in six times. Balb/c mice were inoculated with murine A20 lymphoma cells and when tumor was palpable, the mice were treated with ironomycin (3 mg/kg i.p.). Mice were sacrificed when tumor volume reached 1,500 mm3. Evaluation of the tumor volume of vehicle (n ¼ 10) and ironomycin-treated mice (n ¼ 10). , P < 0.05 using Mann– Whitney test. E, Effect of Ironomycin on LIP was measured by dequenching of Calcein. Cells were loaded with 0.25 mmol/L Calcein-AM for 15 min, washed, and incubated for 1 hour with or without 100 mmol/L deferasirox or 10 mmol/L ironomycin. In this experiment, following treatment, cells were washed and their fluorescence was measured by flow cytometry. The difference in the mean fluorescence index between chelator-treated and untreated cells (DF) is presented. The results represent the mean and SD of at least four independent experiments.
Dlbcl, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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u 2973  (DSMZ)
92
DSMZ u 2973
Figure 2. Iron chelators and ironomycin inhibit <t>DLBCL</t> <t>cell</t> growth and decrease the LIP. A–C, DLBCL cells were incubated with different concentrations of iron chelators or vehicle for 96 hours. IC50 was calculated with concentration–response curve after treatment with deferoxamine (A), deferasirox (B), and ironomycin (C). D, Cell viability was examined using quantification of ATP assay. Data are expressed as mean percentage SEM of at least three independent experiments performed in six times. Balb/c mice were inoculated with murine A20 lymphoma cells and when tumor was palpable, the mice were treated with ironomycin (3 mg/kg i.p.). Mice were sacrificed when tumor volume reached 1,500 mm3. Evaluation of the tumor volume of vehicle (n ¼ 10) and ironomycin-treated mice (n ¼ 10). , P < 0.05 using Mann– Whitney test. E, Effect of Ironomycin on LIP was measured by dequenching of Calcein. Cells were loaded with 0.25 mmol/L Calcein-AM for 15 min, washed, and incubated for 1 hour with or without 100 mmol/L deferasirox or 10 mmol/L ironomycin. In this experiment, following treatment, cells were washed and their fluorescence was measured by flow cytometry. The difference in the mean fluorescence index between chelator-treated and untreated cells (DF) is presented. The results represent the mean and SD of at least four independent experiments.
U 2973, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The reciprocal repression effect of LINC00908 and miR-671-5p. ( A ) Subcellular fractionation assay was used to determine the subcellular localization of LINC00908. ( B ) Expression levels of miR-671-5p in U2932 and FARAGE cells after the knockdown of LINC00908. ( C ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p mimic. ( D ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p inhibitor. ( E ) Expression of miR-671-5p in 28 DLBCL tissues and paired normal tissues based on qRT-PCR. ( F ) The association between LINC00908 and miR-671-5p was examined. ( G ) Expression of miR-671-5p in the GB12878 cells and DLBCL cell lines based on qRT-PCR. **p < 0.01.

Journal: Cancer Management and Research

Article Title: LINC00908 Promotes Diffuse Large B-Cell Lymphoma Development by Down-Regulating miR-671-5p

doi: 10.2147/CMAR.S299715

Figure Lengend Snippet: The reciprocal repression effect of LINC00908 and miR-671-5p. ( A ) Subcellular fractionation assay was used to determine the subcellular localization of LINC00908. ( B ) Expression levels of miR-671-5p in U2932 and FARAGE cells after the knockdown of LINC00908. ( C ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p mimic. ( D ) qPCR analysis of LINC00908 after cells were transfected with miR-671-5p inhibitor. ( E ) Expression of miR-671-5p in 28 DLBCL tissues and paired normal tissues based on qRT-PCR. ( F ) The association between LINC00908 and miR-671-5p was examined. ( G ) Expression of miR-671-5p in the GB12878 cells and DLBCL cell lines based on qRT-PCR. **p < 0.01.

Article Snippet: Human lymphoblastoid B cell (GM12878) and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC).

Techniques: Fractionation, Expressing, Knockdown, Transfection, Quantitative RT-PCR

Figure 2. Iron chelators and ironomycin inhibit DLBCL cell growth and decrease the LIP. A–C, DLBCL cells were incubated with different concentrations of iron chelators or vehicle for 96 hours. IC50 was calculated with concentration–response curve after treatment with deferoxamine (A), deferasirox (B), and ironomycin (C). D, Cell viability was examined using quantification of ATP assay. Data are expressed as mean percentage SEM of at least three independent experiments performed in six times. Balb/c mice were inoculated with murine A20 lymphoma cells and when tumor was palpable, the mice were treated with ironomycin (3 mg/kg i.p.). Mice were sacrificed when tumor volume reached 1,500 mm3. Evaluation of the tumor volume of vehicle (n ¼ 10) and ironomycin-treated mice (n ¼ 10). , P < 0.05 using Mann– Whitney test. E, Effect of Ironomycin on LIP was measured by dequenching of Calcein. Cells were loaded with 0.25 mmol/L Calcein-AM for 15 min, washed, and incubated for 1 hour with or without 100 mmol/L deferasirox or 10 mmol/L ironomycin. In this experiment, following treatment, cells were washed and their fluorescence was measured by flow cytometry. The difference in the mean fluorescence index between chelator-treated and untreated cells (DF) is presented. The results represent the mean and SD of at least four independent experiments.

Journal: Cancer research

Article Title: Targeting Cellular Iron Homeostasis with Ironomycin in Diffuse Large B-cell Lymphoma.

doi: 10.1158/0008-5472.CAN-21-0218

Figure Lengend Snippet: Figure 2. Iron chelators and ironomycin inhibit DLBCL cell growth and decrease the LIP. A–C, DLBCL cells were incubated with different concentrations of iron chelators or vehicle for 96 hours. IC50 was calculated with concentration–response curve after treatment with deferoxamine (A), deferasirox (B), and ironomycin (C). D, Cell viability was examined using quantification of ATP assay. Data are expressed as mean percentage SEM of at least three independent experiments performed in six times. Balb/c mice were inoculated with murine A20 lymphoma cells and when tumor was palpable, the mice were treated with ironomycin (3 mg/kg i.p.). Mice were sacrificed when tumor volume reached 1,500 mm3. Evaluation of the tumor volume of vehicle (n ¼ 10) and ironomycin-treated mice (n ¼ 10). , P < 0.05 using Mann– Whitney test. E, Effect of Ironomycin on LIP was measured by dequenching of Calcein. Cells were loaded with 0.25 mmol/L Calcein-AM for 15 min, washed, and incubated for 1 hour with or without 100 mmol/L deferasirox or 10 mmol/L ironomycin. In this experiment, following treatment, cells were washed and their fluorescence was measured by flow cytometry. The difference in the mean fluorescence index between chelator-treated and untreated cells (DF) is presented. The results represent the mean and SD of at least four independent experiments.

Article Snippet: Human DLBCL cell lines The 16 DLBCL cell lines (U2932, OCI-LY-3, NU-DHL-1, OCI-LY19, DB, SUDHL4, OCILY1, SUDHL5, DOHH2, SUDHL10, HT, RI-1, SU-DHL-6, NUDUL-1, WSU-DLCL-2 and OCI-LY-7) were purchased from theDSMZ (Leibniz-Institut DSMZ -Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH).

Techniques: Incubation, Concentration Assay, ATP Assay, MANN-WHITNEY, Cytometry

Figure 4. Iron chelators and ironomycin induce ROS production in DLBCL cells. A and B, Effect of deferoxamine, deferasirox, and ironomycin on the ROS production was analyzed using flow cytometry after oxidation of a CM-H2DFDA probe for OCI-LY3 (A) and DB cell line (B) at 48 hours. C, Vehicle and GSH treatment (5 and 0.5 mmol/L, respectively, 1 hour) was used as a negative control and oxygen peroxide (10 mmol/L, 1 hour) as a positive control. For the ROS reversion assay, DB cells were treated with 250 nmol/L Ironomycin for 48 hours with or without GSH or Fer-1 or deferoxamine (DFO). ROS production was analyzed using flow cytometry after oxidation of a CM-H2DFDA probe. Chaetocin and erastin were used as positive control for GSH and Fer-1 reversion, respectively. Results represent the mean percentage and SD of three independent experiments. , P < 0.05; , P < 0.01; , P < 0.001; , P < 0.0001; NS, nonsignificant; with paired Student t test.

Journal: Cancer research

Article Title: Targeting Cellular Iron Homeostasis with Ironomycin in Diffuse Large B-cell Lymphoma.

doi: 10.1158/0008-5472.CAN-21-0218

Figure Lengend Snippet: Figure 4. Iron chelators and ironomycin induce ROS production in DLBCL cells. A and B, Effect of deferoxamine, deferasirox, and ironomycin on the ROS production was analyzed using flow cytometry after oxidation of a CM-H2DFDA probe for OCI-LY3 (A) and DB cell line (B) at 48 hours. C, Vehicle and GSH treatment (5 and 0.5 mmol/L, respectively, 1 hour) was used as a negative control and oxygen peroxide (10 mmol/L, 1 hour) as a positive control. For the ROS reversion assay, DB cells were treated with 250 nmol/L Ironomycin for 48 hours with or without GSH or Fer-1 or deferoxamine (DFO). ROS production was analyzed using flow cytometry after oxidation of a CM-H2DFDA probe. Chaetocin and erastin were used as positive control for GSH and Fer-1 reversion, respectively. Results represent the mean percentage and SD of three independent experiments. , P < 0.05; , P < 0.01; , P < 0.001; , P < 0.0001; NS, nonsignificant; with paired Student t test.

Article Snippet: Human DLBCL cell lines The 16 DLBCL cell lines (U2932, OCI-LY-3, NU-DHL-1, OCI-LY19, DB, SUDHL4, OCILY1, SUDHL5, DOHH2, SUDHL10, HT, RI-1, SU-DHL-6, NUDUL-1, WSU-DLCL-2 and OCI-LY-7) were purchased from theDSMZ (Leibniz-Institut DSMZ -Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH).

Techniques: Cytometry, Negative Control, Positive Control

Figure 7. Ironomycin presents a significant toxicity on DLBCL primary cells and potentializes doxorubicin cytotoxicity. A, Primary DLBCL cells were treated with ironomycin and/or doxorubicin and incubated during 96 hours with CD40L. DLBCL cell toxicity was analyzed by flow cytometry. B, Hematopoietic progenitor CFU assay were performed with CD34þ cells from apheresis of 5 donors. Cells were cultured in hydroxyl-methyl-cellulose medium with or without conventional chemotherapy or ironomycin. CFU-C, CFU-E, and CFU-GM were counted after 14-day culture. C, The toxicity of ironomycin alone or in combination with doxorubicin on normal CD3þ cells was assessed by flow cytometry. Results represent the median interquartile ranges of each population (n ¼ 5 patients). Statistical significance was tested using t test of pairs: , P < 0.05; , P < 0.01; , P < 0.001; , P < 0.0001. D, Primary DLBCL cells of 6 patients and PBMC of a healthy donor were incubated with 5 mmol/L Rho-Nox1 Fe2þ probe. The MFI was assessed by flow cytometry in CD20þ DLBCL cells and CD20 cells (microenvironment cells). E, DB cells were treated with increasing concentrations of ironomycin com- bined with doxorubicin for 96 hours, and cell viability was tested by ATP quantification to obtain the viability matrix. The synergy matrix was calculated as described in Materials and Methods.

Journal: Cancer research

Article Title: Targeting Cellular Iron Homeostasis with Ironomycin in Diffuse Large B-cell Lymphoma.

doi: 10.1158/0008-5472.CAN-21-0218

Figure Lengend Snippet: Figure 7. Ironomycin presents a significant toxicity on DLBCL primary cells and potentializes doxorubicin cytotoxicity. A, Primary DLBCL cells were treated with ironomycin and/or doxorubicin and incubated during 96 hours with CD40L. DLBCL cell toxicity was analyzed by flow cytometry. B, Hematopoietic progenitor CFU assay were performed with CD34þ cells from apheresis of 5 donors. Cells were cultured in hydroxyl-methyl-cellulose medium with or without conventional chemotherapy or ironomycin. CFU-C, CFU-E, and CFU-GM were counted after 14-day culture. C, The toxicity of ironomycin alone or in combination with doxorubicin on normal CD3þ cells was assessed by flow cytometry. Results represent the median interquartile ranges of each population (n ¼ 5 patients). Statistical significance was tested using t test of pairs: , P < 0.05; , P < 0.01; , P < 0.001; , P < 0.0001. D, Primary DLBCL cells of 6 patients and PBMC of a healthy donor were incubated with 5 mmol/L Rho-Nox1 Fe2þ probe. The MFI was assessed by flow cytometry in CD20þ DLBCL cells and CD20 cells (microenvironment cells). E, DB cells were treated with increasing concentrations of ironomycin com- bined with doxorubicin for 96 hours, and cell viability was tested by ATP quantification to obtain the viability matrix. The synergy matrix was calculated as described in Materials and Methods.

Article Snippet: Human DLBCL cell lines The 16 DLBCL cell lines (U2932, OCI-LY-3, NU-DHL-1, OCI-LY19, DB, SUDHL4, OCILY1, SUDHL5, DOHH2, SUDHL10, HT, RI-1, SU-DHL-6, NUDUL-1, WSU-DLCL-2 and OCI-LY-7) were purchased from theDSMZ (Leibniz-Institut DSMZ -Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH).

Techniques: Incubation, Cytometry, Colony-forming Unit Assay, Cell Culture

Figure 8. Ironomycin induces mortality and TFR1 downregulation in primary DLBCL cells. A, Primary DLBCL cells from four patients were cultured with recombinant CD40 L in the presence or absence of ironomycin (500 nmol/L). After 96 hours, cell death of DLBCL cells was monitored by Annexin V/PI staining using flow cytometry. Results represent the mean SD. B, Primary samples of patients with DLBCL were cultured with recombinant CD40 L in presence or absence ironomycin (500 nmol/L). DLBCL cell membrane TFR1 (CD71) expression was assessed by flow cytometry. , P < 0.05; , P < 0.01.

Journal: Cancer research

Article Title: Targeting Cellular Iron Homeostasis with Ironomycin in Diffuse Large B-cell Lymphoma.

doi: 10.1158/0008-5472.CAN-21-0218

Figure Lengend Snippet: Figure 8. Ironomycin induces mortality and TFR1 downregulation in primary DLBCL cells. A, Primary DLBCL cells from four patients were cultured with recombinant CD40 L in the presence or absence of ironomycin (500 nmol/L). After 96 hours, cell death of DLBCL cells was monitored by Annexin V/PI staining using flow cytometry. Results represent the mean SD. B, Primary samples of patients with DLBCL were cultured with recombinant CD40 L in presence or absence ironomycin (500 nmol/L). DLBCL cell membrane TFR1 (CD71) expression was assessed by flow cytometry. , P < 0.05; , P < 0.01.

Article Snippet: Human DLBCL cell lines The 16 DLBCL cell lines (U2932, OCI-LY-3, NU-DHL-1, OCI-LY19, DB, SUDHL4, OCILY1, SUDHL5, DOHH2, SUDHL10, HT, RI-1, SU-DHL-6, NUDUL-1, WSU-DLCL-2 and OCI-LY-7) were purchased from theDSMZ (Leibniz-Institut DSMZ -Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH).

Techniques: Cell Culture, Recombinant, Staining, Cytometry, Membrane, Expressing